Antibacterial and anti-biofilm studies of synthetic compounds derived from marine antimicrobial compounds

P35 Antibacterial and anti-biofilm studies of synthetic compounds derived from marine antimicrobial compounds

Ekatarina Mishchenko et al.

P35 Antibacterial and anti-biofilm studies of synthetic compounds derived from marine antimicrobial compounds

Hospital-acquired wound infections are of increasing medical concern due to their leading positions in morbidity and treatment difficulties. In this respect studying novel marine compounds with specific mechanism of action is important and has a potential value for development of new treatment strategies for these infections. In a MabCent PhD project, we performed antibacterial screening of synthetic compounds based on scaffolds found in marine bioactive compounds. Almost half of the tested compounds showed inhibitory activity in a micro-dilution bacterial growth inhibition assay, and 1/5 of them were active against both Gram-positive and Gram-negative bacteria tested. These results were followed up by anti-biofilm assays using a microtiter plate method. Weak inhibitory activity against S. epidermidis biofilms was revealed. Compounds showing strong antimicrobial effect were able to eliminate up to 70 % of the biofilm. In order to determine whether active compounds act by membrane interference, we used a membrane integrity assay with luciferase expressing bacteria species. While membrane disruption was observed for some compounds in Gram-positive bacteria, the same compounds presumably affect intracellular targets in Gram-negative microbes. Development of novel in vitro reporter assays to characterize anti-biofilm activity is going on and further results will be shown at the conference.

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