p1 Metabolomics as a Tool in the Identification and Production of New Antibiotics from Sponges and Endosymbiotic Bacteria (selected for oral presentation)
Metabolomic methods can be utilised to screen diverse biological systems for potentially novel and sustainable sources of antibiotics and pharmacologically-active drugs. Marine spong- es and their endosymbionts have proven to be abundant in bioactive compounds. HR-LCFTMS and NMR were used in the identification of compounds isolated from a bacteria and its host sponge, as well as in the dereplication and metabolic profiling of other sponge-associated bacteria.
24-methylenecholesterol and one novel ster- oid, both significantly active against Trypa- nosoma brucei, were isolated from the Irish Sea sponge Haliclona simulans. Extracts from Streptomyces sp. isolated from H. simulans demonstrate anti-bacterial, anti-fungal and
anti-calcineurin activities. HR-LCFTMS assisted in identifying antimycins as the anti-fungal com- pounds. NMR and HR-LCFTMS were applied to the dereplication of extracts from bacteria from Mediterranean sponges. EG4 was selected and its cultivation optimised from small scale to larg- er scale production with the aid of metabolomic methods to identify and trace biomarkers.
Metabolomics has become a powerful tool in systems biology which allows us to gain insights into the potential of natural marine isolates for synthesis of significant quantities of promising new agents, and allows us to manipulate the environment within fermentation systems in a rational manner to select a desired metabolome.
Presentation not available