P13 Isolation and Characterization of Secondary Metabolites from Extracts of Arctic Marine Invertebrates

P13 Isolation and Characterization of Secondary Metabolites from Extracts of Arctic Marine Invertebrates

Eirin Glomsaker1, Espen Hansen1, Jeanette H. Andersen1, Johan Isaksson2and Kine Ø. Hansen1

1Norwegian College Of Fishery Science, Uit - The Arctic University Of Norway, Tromsø, NOR 2Department Of Chemistry, Uit - The Arctic University Of Norway, Tromsø, NOR

Marine invertebrates are known to produce a wide variety of secondary metabolites, many of which have interesting bioactivities. The aim of this study was to isolate and characterize secondary metabolites from extracts of eight Arctic marine invertebrates. Crude aqueous and organic extracts prepared from these collected organisms were prefractionated using flash chromatography. Anticancer screening was conducted on prefractionated fractions. Active fractions were retested to confirm the bioactivity and to prioritize fractions for dereplication.

Fractions with confirmed bioactivity were dereplicated using UHPLC-HR-MS to investigate if the compounds present in the active fraction were novel or if the compound had previously been reported. Compounds from two different invertebrate extracts that were believed to be novel were isolated using prep-HPLC-MS. This resulted in pure compounds whose structures were elucidated using HR-MS and NMR. Further bioactivity testing was conducted on these pure compounds. A selection of these results will be presented.