P14 Drug Discovery in Petri Dish against Crop Diseases: Microbial Co-cultivation for Increasing the Chemical Diversity of Marine Fungi

P14 Drug Discovery in Petri Dish against Crop Diseases: Microbial Co-cultivation for Increasing the Chemical Diversity of Marine Fungi

Ernest Oppong-Danuah1, Delphine Parrot1, Antje Labes2 and Deniz Tasdemir1

1GEOMAR Centre For Marine Biotechnology (GEOMAR-Biotech), Research Unit Marine Natural Products Chemistry, GEOMAR Helmholtz Centre For Ocean Research Kiel, Kiel, DEU
2Flensburg University Of Applied Sciences, Flensburg, DEU

Crop diseases reduce agricultural yield significantly, contributing to poverty and hunger worldwide. Due to resistance development, there is a need for discovering new, more effective biocontrol agents. Marine fungi represent a rich source for bioactive metabolites. However, the gene clusters of large proportions of the cultivable fungi remain silent in standard laboratory culture conditions. Co-cultivation has been used to awaken biosynthetic pathways to produce cryptic compounds. We have isolated 120 fungal strains from various sources from the Baltic Sea. The fungi were directly co-cultivated with a panel of phytopathogens. The extracts of both mono- and co-cultures were analysed using UPLC-QTOF-MS/MS and their bioactivities tested. Metabolomics and molecular networking using GNPS platform were applied for dereplication/annotation of the chemical metabolites. This work will describe our efforts for stimulation of chemical capacity and bioactivity of the most promising co-culture pairs for bioactivity-guided purification scheme.