P19 Effect of co-culturing on the antibiotic activity of marine fungi

P19 Effect of co-culturing on the antibiotic activity of marine fungi

Teppo Rämä1, Venke Kristoffersen1, Jeanette H. Andersen1 and Espen Hansen1

1Marbio, Uit - The Arctic University Of Norway, Tromsø, NOR

Relatively few marine fungi, which are studied for their production of secondary metabolites, represent species that are restricted to marine habitats. The application of the OSMAC approach on these marine fungi sensu stricto (s.s.) has demonstrated that they are versatile producers of new and known secondary metabolites. However, the effect of bacterial co-culturing on secondary metabolite production of marine fungi s.s. has not been assessed. In this experimental study, we tested the effect of co-culturing on the antibacterial activity of marine fungi based ona 34-isolate dataset. The isolates were fermented in co-culturing and non-co-culturing treatments and FLASH fractions of the fermentation extracts were screened in antibacterial assays. The responses of fungi to the co-culturing varied. More than one third of the fungal isolates showed induced or enhanced production of antibacterial metabolites in the co-culturing treatment. Some of the metabolites detected are putatively new.