P21 Development of a novel expression host for challenging enzymes

P21 Development of a novel expression host for challenging enzymes

Jenny Söderberg1, Miriam Grgic1 and Peik Haugen1

1Department Of Chemistry , The Artic University Of Norway Uit, Tromsø, NOR

Successful expression and purification of enzymes represents a major challenge, in particular psychrophilic (cold adapted) enzymes are difficult to express in standard mesophilic hosts (e.g. E. coli). This group of enzymes are commercially and economically relevant due to their unique properties. However, the lack of specialised expression systems continue to hamper development of new psychrophilic enzymes to the market. Typically, low expression, misfolded/non-functional protein and inclusion bodies hinder successful protein production.

We have recently identified bacterial isolates that are promising hosts for expressing psychrophilic and challenging proteins. We have performed basic characterization such as growth at different temperatures, up take of genetic material and expression of fluorescent (reporter) proteins from plasmids. Interestingly, one isolate was highly suitable as an expression host. We are currently working with difficult enzyme targets that has shown to fail when expressed in E.coli.