P22 Antimicrobial peptides in the marine gastropod, Neptunea despecta

P22 Antimicrobial peptides in the marine gastropod, Neptunea despecta

Lindsey Leigh Beecher Martinsen1, Ida Kristine Hansen1, Klara Stensvåg1 and Tor Haug1

1The Norwegian College Of Fishery Science, Faculty Of Biosciences, Fisheries And Economics, Uit The Arctic University Of Norway, Tromsø, NOR

Bacterial resistance towards commercial antibiotics is one of the most serious threats to human health today. It is therefore an urgent need to develop new types of antimicrobials with different modes of action. Antimicrobial peptides (AMPs) are an important part of the natural defence system against invading pathogenic microorganisms in all types of invertebrates studied. AMPs kill microorganisms effectively and are therefore considered to be promising candidates for drug development. The aim of this master project is to search for AMPs in different tissues of the marine mollusc Neptunea despecta. Freeze-dried samples were subjected to solvent extraction, partitioning, and solid phase extraction (SPE) on C18 cartridges. The SPE-eluates were subsequently tested for antibacterial activity against Escherichia coli and Corynebacterium glutamicum. Antibacterial activity as detected in several tissue extracts, including the haemocytes. AMPs were detected and separated using high performance liquid chromatography (HPLC) and partially characterized with high-resolution mass spectrometry (HR-MS).