P28 The Osteogenic Potential of Brown Seaweeds Extracts

P28 The Osteogenic Potential of Brown Seaweeds Extracts

Aaron O'kane1, Matthew Carson2, Susan Clarke2, Paulo J. Gavaia3, Vincent Laizé4, Leonor Cancela3, Gary Sheldrake1 and Pamela Walsh1

1Chemistry &Chemical Engineering, Queen's University Belfast, Belfast, GBR

2Nursing And Midwifery, Queen's University Belfast, Belfast, GBR
3Centre Of Marine Sciences (CCMAR), Department Of Biomedical Sciences And Medicine And Algarve Biomedical Centre, University Of Algarve, Faro, PRT
4Centre Of Marine Sciences (CCMAR), University Of Algarve, Faro, PRT

Brown seaweeds are evolutionarily and chemically distinct from land plants and offer significantly higher biodiversity. They produce secondary metabolites, with novel chemical signatures that cannot be found in other organisms. The osteogenic potential of compounds extracted from Ascophyllum nodosum using conventional organic solvents and green extraction technology was tested in a zebrafish system. Zebrafish exhibit a skeleton similar to humans, that can remodel (i.e. it contains osteoblasts, osteoclast and osteocytes)1. In this experiment, zebrafish larvae were used to evaluate the ability of A. nodosum extracts to stimulate opercular bone growth. The concentration of A. nodosum extracts tested ranged between 3-23 µg/ml. NMR spectroscopy and GC-MS were used to characterise the extracts. Preliminary results found that fatty acids extracts (mainly oleic and linoleic acid) which were isolated, using supercritical carbon dioxide as the extractive solvent, had the greatest osteogenic effect.

1. Montazerolghaem, M., et al. Acta Biomater. 19, 10–14(2015).