1Blue Biotechnology And Ecotoxicology Laboratory, Interdisciplinary Centre Of Marine And Environmental Research - University Of Porto, Porto, PRT
2Blue Biotechnology And Ecotoxicology, Interdisciplinary Centre Of Marine And Environmental Research - University Of Porto, Porto, PRT
3Centre Of Health And Environmental Research - Polythechnic Institute Of Porto, Porto, PRT
4Department Of Oncology And Pathology, Karolinska Institute, Stockholm, SWE
5 Faculty Of Sciences Of The University Of Porto, Porto, PRT
6 Blue Biotechnology Laboratory, Interdisciplinary Centre Of Marine And Environmental Research - University Of Porto, Porto, PRT
In the presented work,three compounds and 92 chromatographic fractions obtained from crude organic extracts of 28 cyanobacterial strains from the Portuguese coast were tested on multicellular spheroids generated from the human cell lines HCT116,HT-29 and A549. Spheroids were incubated and analysed in an IncuCyte®ZOOM System,and M30 Cytodeath ELISA was performed for apoptosis. Portoamides isolated from Oscillatoria sp.LEGE05292 reduced HCT116 spheroid integrity. Bartoloside B from Synechocystis salinaLEGE06155 decreased the size of HCT116 spheroids. Compound A isolated from Nodularia sp.LEGE06071 induced a loss of the boundaries of the spheroid. Beyond that, portoamides induced apoptosis on HCT116 and HT29 spheroids, and compound A on HCT116 and A549 spheroids. The screening revealed that five fractions reduced the spheroid integrity; isolation of the active components will be done in the future by bioassay-guided fractionation. We conclude that the tested compounds and extracts show biological activities on multicellular spheroids and are therefore interesting candidates to be further developed as therapeutics for solid tumors.