P35 Discovery of proteases for bioconversion of marine biomass

P35 Discovery of proteases for bioconversion of marine biomass

Øivind Larsen1, Hasan Arsın2, Runar Stokke2, Antonio García-Moyano1, Pål Puntervoll1, Ingar Leiros3, Arne O. Smalås3, Ida H. Steen2, Gro Bjerga1

1 Uni Research, Center for applied biotechnology, Thormøhlens gt 55, 5006 Bergen, Norway; 2 University of Bergen, Department of Biology, Post box 7803, N-5020 Bergen, Norway; 3 University of Tromsø, Department of Chemistry, Research Park 3, Sykhusvegen 23, 9019 Tromsø, Norway.

New enzymes will be key to future developments and value creation in the emerging marine bio-based industry. The Norwegian fishing industry generates 890 000 tons by-products, such as heads and frames annually. This is mainly used in animal feed, oil and fish silage, or discarded. The by-products contain high-quality proteins and have a great potential as high-value products for human consumption. The few commercial enzymes used for fish hydrolysis are, however, not optimal. Together with the Norwegian ingredient industry and academic partners we utilized various strategies to identify new proteases for the marine bio-based industry. Using (meta)genomic mining new proteolytic enzymes were identified on a panel of substrates. The genes originate from in situ enrichments at deep-sea hot vent systems and from Arctic, marine microbial species. In-depth characterization and structural studies of enzymes are ongoing and their performance in hydrolysis of marine biomass at pilot scale is currently evaluated.