1GEOMAR Centre For Marine Biotechnology (GEOMAR-Biotech) And Research Unit Marine Natural Products Chemistry, GEOMAR Helmholtz Centre For Ocean Research Kiel, Kiel, DEU
2Department Of Energy And Biotechnology, University Of Applied Sciences Flensburg, Flensburg, Flensburg, DEU
3Research Unit Experimental Ecology - Benthic Ecology, GEOMAR Helmholtz Centre For Ocean Research Kiel, Kiel, DEU
4Department Of Biology, Institute For General Microbiology, Kiel University, Kiel, DEU
Desorption electrospray ionisation (DESI) enables the ambient ionisation of natural products, without previous processing of analytes. The combination of DESI with imaging mass spectrometry (IMS) allows analysis of the spatial localisation and molecular distribution of target molecules on surfaces and within tissues of natural sources. In contrast to matrix assisted laser desorption ionisation (MALDI)-IMS, DESI-IMS is a matrix-free, neutral ionisation technique with minimal sample preparation requirements. DESI-IMS that detects low m/z ions via a soft ionisation of analytes has applicability to various types of natural products, hence allowing the collection of a wealth of critical molecular information. The extraction-free in situ analysis of natural products makes DESI-IMS a powerful tool for modern natural product research. At GEOMAR-Biotech DESI-IMS is employed for identification of secondary metabolites from surfaces of marine resources in combination with metabolite profiling/metabolomics and marine natural product chemistry from a chemical ecology perspective and a drug discovery angle.