High-throughput functional metagenomics for the discovery of glycan metabolic pathways

Thursday March 9
Parallel Session 3B: Synthetic biology – Enabling targeted and accelerated production organism development
14:15

High-throughput functional metagenomics for the discovery of glycan metabolic pathways

Gabrielle Potocki-Veronese, Research Director
INRA, France

Research by: Gabrielle Potocki-Veronese, Elisabeth Laville, Simon Ladeveze, Alexandra Tauzin, Sandra Pizzut-Serin, Patrick Robe, Marion Leclerc & Bernard Henrissat

In most of microbial ecosystems, bacteria and fungi produce a full repertoire of carbohydrate active enzymes to face the huge structural diversity of carbohydrates which constitute their main carbon sources. A high-throughput metaomic approach was developed to guide the in-depth sequencing of specific regions of metagenomes and metatranscriptomes involved in the breakdown of complex carbohydrates. Integration of biochemical, structural, meta-omic and omic data at the level of the targeted ecosystems revealed novel mechanisms of plant, microbial and mammal glycan metabolization, involving a battery of glycoside-hydrolases, - phosphorylases and sugar transporters. Thanks to these data, these fascinating proteins now appear as new targets to study interactions between prokaryotes and eukaryotes, and also constitute efficient tools for biotechnology.