Tiago Silva (Universidade do Minho, Portugal) - A wave moving marine materials up to the biomedical field: engineering different human tissues as high added-value blue biotechnology approach
Tiago H. Silva1and Rui L. Reis1
13B's Research Group, University Of Minho, Guimarães, PRT
Marine organisms synthesize a plethora of chemical compounds with vast biological activity. In many occasions, such compounds organize themselves in such particular architectures that the resulting structures exhibit astonishing features regarding mechanical or biological performance. Thus, marine environment has been a remarkable source of inspiration for scientists and engineers, including the ones developing innovative biomaterials to promote the regeneration of different human tissues. Besides, other functional materials are also being proposed with biomedical application. In this perspective, several examples being developed in our lab will be discussed, namely (i) squid chitosan, fucoidan and marine collagens for scaffolding and cell encapsulation; (ii) fucoidan exhibiting anti-tumor activity over breast cancer cells, depending on chemical features; (iii) dynamic collagenous tissues as inspiration for the development of functional and smart biomaterials; and (iv) marine sponges architectures as potential Naturemade scaffolds.
Giovanna Romano (Stazione Zoologica Anton Dohrn, Italy) - Actiskin: a new cosmeceutical formulation from the green microalga Tetraselmis suecica
Christian Galasso1, Clementina Sansone2, Angelo Fontana3, Genoveffa Nuzzo4, Adele Cutignano4, Tiziano Croci5, Rolando Lorenzetti5, Giovanna Romano6 and Adrianna Ianora2
1Integrative Marine Ecology, Stazione Zoologica Anton Dohrn, Naples, ITA
2Integrative Marine Ecology, Stazione Zoologica Anton Dohrn/Biosearch Srl, Naples, ITA
3ICB-CNR /Biosearch Srl, Pozzuoli, ITA
4ICB-CNR/Biosearch Srl, Pozzuoli, ITA
5Biosearch Srl, Milan, ITA
6Stazione Zoologica Anton Dohrn/Biosearch Srl, Naples, ITA
The present invention relates to the biological activity of an alcohol/water extract of the marine green microalga containing many active pigments and substances such as carotenoids having antioxidant, protective and repairing activity on human cells. Thus, the invention relates to the use of said extract in cosmetic compositions, neutraceutical supplements, medical compositions. Green microalgae contain many active pigments such as carotenoids having antioxidant and protective activity on human cells. This extract has a strong antioxidant and repairing activity in the human cell line (A549) reducing the expression of dehydrocholesterol reductase-24 (DHCR24) and prostaglandin reductase 1 (PTGR1) genes and proteins. The extract also reduces prostaglandin E2 (PGE2) levels in cells damaged by H2O2 and has tissue repairing effects on human epidermal tissue model (EpiDermTM) indicating a potential cosmeceutical activity of this microalgal species.
Pamela Walsh (Queen's University Belfast, Ireland) - The Osteogenic Potential of Brown Seaweeds Extracts
Aaron O'kane1, Matthew Carson2, Susan Clarke2, Paulo J. Gavaia3, Vincent Laizé4, Leonor Cancela3, Gary Sheldrake1 and Pamela Walsh1
1Chemistry &Chemical Engineering, Queen's University Belfast, Belfast, GBR
2Nursing And Midwifery, Queen's University Belfast, Belfast, GBR
3Centre Of Marine Sciences (CCMAR), Department Of Biomedical Sciences And Medicine And Algarve Biomedical Centre, University Of Algarve, Faro, PRT
4Centre Of Marine Sciences (CCMAR), University Of Algarve, Faro, PRT
Brown seaweeds are evolutionarily and chemically distinct from land plants and offer significantly higher biodiversity. They produce secondary metabolites, with novel chemical signatures that cannot be found in other organisms. The osteogenic potential of compounds extracted from Ascophyllum nodosum using conventional organic solvents and green extraction technology was tested in a zebrafish system. Zebrafish exhibit a skeleton similar to humans, that can remodel (i.e. it contains osteoblasts, osteoclast and osteocytes)1. In this experiment, zebrafish larvae were used to evaluate the ability of A. nodosum extracts to stimulate opercular bone growth. The concentration of A. nodosum extracts tested ranged between 3-23 µg/ml. NMR spectroscopy and GC-MS were used to characterise the extracts. Preliminary results found that fatty acids extracts (mainly oleic and linoleic acid) which were isolated, using supercritical carbon dioxide as the extractive solvent, had the greatest osteogenic effect.
1. Montazerolghaem, M., et al. Acta Biomater. 19, 10–14(2015).